AOAC ERP MICRO AUGUST 2018

AOAC Official Methods Program  Expert Review Panel for Microbiology for Foods and Environmental Surfaces 

OMA 2016.07: DETECTION OF LISTERIA SPECIES IN SELECT FOODS AND ENVIRONMENTAL SURFACES

Was the method's applicability considered?

ER 1 ER 2 ER 3 ER 4 ER 5

Yes Yes Yes Yes Yes

Please state the method's applicability statement:

ER 1 Applicable to the detection of Listeria species in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage  cheese (25 g), vanilla ice cream (25 g), queso fresco (25 g), spinach (25 g), melon (whole), raw chicken leg pieces (25 g), raw  chicken fillet (25 g); and concrete [3M Hydrated Sponge Stick with Dey– Engley (D/E) broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge Stick with D/E broth; 225 mL), and plastic (3M  Enviro Swab with Letheen; 10 mL) environmental samples.  ER 2 Select Foods and Environmental Surfaces ER 3 Applicable to the detection of Listeria species in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage  cheese (25 g), vanilla ice cream (25 g), queso fresco (25 g), spinach (25 g), melon (whole), raw chicken leg pieces (25 g), raw  chicken fillet (25 g); and concrete [3M Hydrated Sponge Stick with Dey–Engley (D/E) broth; 225 and 100 mL], stainless steel  (3M Hydrated Sponge Stick with D/E broth; 225 mL), and plastic (3M Enviro Swab with Letheen; 10 mL) environmental  samples

Applicable to the detection of Listeria monocytogenes in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), queso fresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g), melon (whole), raw chicken leg pieces (25 g), and raw chicken breast fillet (25 g), as well as on sealed concrete [3M Hydrated Sponge with Dey‐Engley (D/E) Neutralizing Broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge with D/E Neutralizing Broth; 225 mL), and plastic (high‐density polyethylene; 3M EnviroSwab with Letheen Broth; 10 mL) environmental samples.

ER 4

ER 5 Applicable to the detection of Listeria species in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), vanilla ice cream (25 g), queso fresco (25 g), spinach (25 g), melon (whole), raw chicken leg pieces (25 g), raw chicken fillet (25 g); and concrete [3M Hydrated Sponge Stick with Dey– Engley (D/E) broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge Stick with D/E broth; 225 mL), and plastic (3M Enviro Swab with Letheen; 10 mL) environmental samples. Were the method's safety concerns addressed prior to the approval for AOAC First Action Official Methods status? ER 1 Yes ER 2 No ER 3 Yes ER 4 Yes ER 5 Yes Is the documentation for the safety evaluation available and completed? ER 1 Yes ER 2 Yes ER 3 No ER 4 Yes ER 5 Yes Are there any reference materials considered or addressed? ER 1 Yes ER 2 No

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