AOAC ERP MICRO AUGUST 2018

AOAC Official Methods Program  Expert Review Panel for Microbiology for Foods and Environmental Surfaces 

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Yes Yes No

Is there documentation regarding reference materials available and noted? If so, where is it located?

P. 98 of Journal of AOAC International Vol. 100, No. 1, 2017

NA

The inclusivity/exclusivity data is in the PTM report.

Reference cultures

No. What type of single laboratory validations were used for this method [i.e, Harmonized PTM, Precollaborative,  Independent, Collaborative, etc.] and where is it referenced in the method documentation? ER 1 Harmonized AOAC Performance Tested MethodsSM (PTM) study. The objective of the PTM study was to demonstrate that  the 3M MDA 2–Listeria method could detect Listeria in a broad range of food matrixes and environmental surfaces as claimed by the manufacturer

Collaborative. AOAC Official Method 2016.07 Detection of Listeria Species in Select Foods and Environmental Surfaces 3M Molecular Detection Assay (MDA) 2–Listeria Method First Action 2016

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Harmonized PTM Harmonized PTM

Performance Tested Method 11150156

Are the approved validation protocols available and documented?

Yes Yes No Yes

Yes Is the method documentation available that demonstrates Reproducibility/Uncertainty and Probability of Detection? If so,  please cite the reference.

STATISTICAL REVIEW: Is the documentation for the statistical review prior to AOAC First Action Official Methods status  available and completed?

yes yes

Not sure where.

Yes

Yes Please cite below the OMA Appendicies, Community Guidance, or  SMPR by which this method was reviewed against. ER 1 U.S. Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) Microbiology Laboratory Guidebook (MLG)  Chapter 8.09 “Isolation and identification of Listeria monocytogenes from red meat, poultry and egg products, and  environmental samples” (5) for deli turkey (125 g) and raw chicken breast fillet (25 g).

yes AOAC Official Method 2016.07 Detection of Listeria Species in Select Foods and Environmental Surfaces 3M Molecular Detection Assay (MDA) 2–Listeria Method First Action 2016

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Appendix J. Appendix J

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