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AOAC First Action Method 2016.02 (Biotin) MLT Report
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TABLE OF CONTENTS
Section
Description
Page
1
Introduction
3
1.1
Study Title
3
1.2
Study Objective
3
1.3
Study Director
3
1.4
Study Monitor
3
1.5
Abbreviations
3
2
The MLT Overview
3
2.1
Method set up and qualification of participants
3
2.2
Multi laboratory testing by qualified participants
4
2.3
Statistical evaluation
4
3
Materials and Methods
4
3.1
AOAC SPIFAN Matrices
4
3.2
Collaborating laboratories
5
3.3
Analytical method
5
4
Results and Discussion
10
4.1
The overview
10
4.2
Linearity and Range
11
4.3
MLT Results and Statistical Evaluation
11
4.4
Relative Standard Deviation of Repeatability (RSDr)
11
4.5
Relative Standard Deviation of Reproducibility (RSDR)
12
4.6
Horwitz Ratio (HorRat)
12
4.7
Accuracy (NIST SRM 1849a)
12
4.8
Selectivity (Comparison with LCMS/MS
12
5
Summary and Conclusion
15
6
Recommendations
15
7
Notes from the Study Director
15
8
References
16
9
Acknowledgements
16
Tables / Figures
Title
Page
Table 1
AOAC SPIFAN Matrices
4
Table 2
MLT Participants
5
Table 3
HPLC Gradient Program
8
Table 4
Retention times and correlation coefficients
11
Table 5
Biotin (µg/100g) Practice Samples
13
Table 6
Biotin (µg/100g) MLT samples (Day 1)
13
Table 7
Biotin (µg/100g) MLT samples (Day 2)
13
Table 8
Statistical evaluation biotin results
14
Table 9
Comparison with LCMS/MS technique
14
Table 10
Comparison of method performance
15
Figure 1
Calibration standard
9
Figure 2
MLT Sample 8B (00859RF00 / PZGP859)
10
2016.02 (FEBRUARY 2017) BIO-02 MLT REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE