SPIFAN Nutrients ERP Book 9-29-15

ples of the above mentioned sample types. No interferences have been ob-

served.

Linearity:

The Linearity has been evaluated twice for both compounds with a 9 points cali-

bration.

For the data and the result of the regression analysis performed in excel, see ap-

pendix 1.

Carnitine:

There is seen a very significant correlation between concentration ratio and area

ratio. R

=0,9998. The slope of the line is 0,9596 and the intercept with the y-axis

is 0,0046. The calculated 95% confidence interval for the intercept includes 0.

The calibration curve therefore goes through 0.

For residuals plotted against the concentration ratio, it is seen that the points are

randomly distributed around the x-axis (concentration ratio), indicating that the

calibration curve for the concentrations included are linear.

Precision:

For the evaluation of the precision two types of samples have been used:



A milk powder (nutritional formula).



A pet food. (dry pet food)

The repeatability (S

) and reproducibility (S

) have been evaluated with a variance

analysis model. The data consists of 16 days of double replicate measurements.

The dataset has been evaluated with ANAVA single factor (Excel 2010), see ap-

pendix 2.

Milk powder (mg/kg)

Pet food (mg/kg)

Carnitine

Reproducibility (S

)

3,1%

3,3%

Repeatability (S

)

2,6%

1,9%

1,865

5,188

crit

2,352

For carnitine in the pet food it is seen that there is a significant difference in the

measured results between days. Considering that a new calibration curve is

Carn-06 - Validation Report

FOR ERP USE ONLY

DO NOT DISTRIBUTE