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Free Choline -OH (mg/kg) on TQD LC-MS/MS, single day
Spike: 100 µL of 593.4 µg/mL choline = 59.3 µg into 3.00g sample of reconstituted 11750017V3. This is 2.0 mg added choline to 100g RTF.
Sample Size: 3.00g of a 10.93% (w/w) reconstitution
Product Name
Lot #
Conc. (OH-)
SPK-60.34 Theor. Spike Recovery Spike Level
mg/kg
mg/kg
mg/kg
mg/100g RTF
SRM1849a
CLC-10b
907.7
Adult Powder-1
11750017V3
60.14
Adult Powder-2
11750017V3
59.76
Adult Powder-3
11750017V3
61.49
Adult Powder-4
11750017V3
60.60
Adult Powder-5
11750017V3
60.87
Adult Powder-6
11750017V3
59.53
Adult Powder-7
11750017V3
59.99
UNSPK. MEAN
60.34
RSD
1.1%
Adult Powder-LOQ-1 11750017V3
233.5
173.1
178.9
96.8%
1.92
Adult Powder-LOQ-2 11750017V3
234.9
174.6
178.6
97.8%
1.94
Adult Powder-LOQ-3 11750017V3
233.3
172.9
176.5
97.9%
1.92
Adult Powder-LOQ-4 11750017V3
232.5
172.2
179.4
96.0%
1.91
Adult Powder-LOQ-5 11750017V3
234.1
173.7
180.7
96.1%
1.93
Adult Powder-LOQ-6 11750017V3
235.5
175.2
179.0
97.9%
1.95
Adult Powder-LOQ-7 11750017V3
232.2
171.8
179.0
96.0%
1.91
SPIKED MEAN
233.7
96.9%
RSD
0.5%
0.9%
Free Carnitine (mg/kg) on TQD LC-MS/MS, single day
Spike: 61 µL of 77.9 µg/mL carnitine = 4.75 µg into 3.00g sample of reconstituted 11750017V3. This is 0.16 mg added carnitine to 100g RTF.
Sample Size: 3.00g of a 10.93% (w/w) reconstitution
Product Name
Lot #
Conc.
SPK-0.0 Theor. Spike Recovery Spike Level
mg/kg
mg/kg
mg/kg
mg/100g RTF
SRM1849a
CLC-10b
130.0
Adult Powder-1
11750017V3
0
Adult Powder-2
11750017V3
0
Adult Powder-3
11750017V3
0
Adult Powder-4
11750017V3
1.1
Adult Powder-5
11750017V3
0
Adult Powder-6
11750017V3
1.3
Adult Powder-7
11750017V3
0
MEAN
0.0
RSD
N/A
Adult Powder-LOQ-1 11750017V3
15.22
15.22
14.33
106.2% 0.169
Adult Powder-LOQ-2 11750017V3
14.95
14.95
14.31
104.5% 0.166
Adult Powder-LOQ-3 11750017V3
14.90
14.90
14.14
105.4% 0.166
Adult Powder-LOQ-4 11750017V3
15.30
15.30
14.37
106.4% 0.170
Adult Powder-LOQ-5 11750017V3
15.00
15.00
14.48
103.6% 0.167
Adult Powder-LOQ-6 11750017V3
15.48
15.48
14.34
108.0% 0.172
Adult Powder-LOQ-7 11750017V3
15.36
15.36
14.34
107.1% 0.171
MEAN
15.17
105.9% 0.169
RSD
1.5%
1.4%
1.5%
RESULTS OF SPIKING FREE CHOLINE AND CARNITINE INTO PRODUCT MATRIX AT THE SMPR
REQUIRED LOQ
Carn-05 MEF Form
FOR ERP USE ONLY
DO NOT DISTRIBUTE