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Abbreviations and synonyms:

Abbreviations:

NMKL.................Nordisk Metodikk Komité for Næringsmidler

ISO ....................International Organization for Standardization

SOP...................Standard Operating Procedure

ANAVA ..............Analysis of Variance

LOD ...................Limit of Detection

LOQ...................Limit of Quantitation

n ........................Number of replicates

LC......................Liquid Chromatography

MS .....................Mass spectroscopy

R

2

.......................Squared regression coefficient

RSD%................Relative Standard Deviation in Percent

CI .......................Confidence intervals

S

r

.......................Repeatability (standard deviation within days)

S

R

......................Reproducibility (standard deviation between days)

SRM ..................Certified reference material

NIST ..................National Institute of Standards and Technology

Synonyms:

t-test ..................

Student’s t

-test

Quality statement:

This validation report is conducted in accordance to our internal instruction

211.01 “Validering af analysemetoder i Vitamincenter

(in English - Validation of

analytical methods in the Vitamin Centre”

This instruction defines the experimental design used in the current validation,

which is based on NMKL Procedure No. 4: Validation of chemical analytical

methods (2009, 3

rd

edition).

The Eurofins Steins Laboratory in Vejen is accredited according to ISO 17025.

Introduction:

This method was developed in the vitamin centre.

The extraction is based on an old internal Eurofins method for determination of

choline.

Carn-06 (Choline Validation Report)

FOR ERP USE ONLY

DO NOT DISTRIBUTE