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Two Different Test Kits
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Encompass antibody differences and extraction differences
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Analyze in triplicate (minimum)
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Multiple Controls
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False Negative
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False Positive
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Dynamic Range
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Determine and identify analyst error
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Additional confirmation testing may be performed
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Multi-allergen antibody detection (profiles)
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DNA (PCR and/or “barcoding”)
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Mass Spectrometry
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Western blots
Allergen Regulatory Testing
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Appropriate Standards for Hydrolyzed and Fermented Foods
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Appropriate Standards for Processed Foods
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Multi-allergen antibody detection (profiles/cross reactivity)
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Measure of Cross Reactivity
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Clear protocols:
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Fortification/Recovery
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Cross Reactivity
Technical Challenges