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Emerging Concepts in Ion Channel Biophysics
Wednesday Speaker Abstracts
25
High-Speed Atomic Force Microscopy (HS-AFM): A New Tool for the Direct Study of
Conformational Changes in Gated Ion Channels
Martina Rangl
1,2
, Yi Ruan
2
, Arin Marchesi
2
, Pierre-Jean Corringer
3
, Crina Nimigean
1
,
Simon
Scheuring
1,2
.
1
Weill Cornell Medicine, New York, NY, USA,
2
INSERM, Marseille, France,
3
Institut Pasteur,
CNRS, Paris, France.
The advent of high-speed atomic force microscopy (HS-AFM1) has opened a novel research
field for the dynamic analysis of single bio-molecules: Molecular motor dynamics2,3 membrane
protein diffusion4, assembly5 and conformational changes of transporters6 could be directly
visualized. Further developments for buffer exchange7 and temperature control8 during HS-
AFM operation provide breakthroughs towards the performance of dynamic structural
biochemistry using HS-AFM. Here, we show the direct visualization of conformational changes
of the cyclic nucleotide gated potassium channels upon ligand binding9, and of a pentameric
receptor ion channel in pH-gating10.
1) Ando et al., Chemical Reviews, 2014, 114(6):3120-88.
2) Kodera et al., Nature, 2010, 468(7320):72-6.
3) Uchihashi et al., Science, 2011, 333(6043):755-8.
4) Casuso et al., Nature Nanotechnology, 2012, 7(8):525-9.
5) Chiaruttini et al., Cell, 2015,163(4):866-79.
6) Ruan et al., PNAS, 2017, doi: 10.1073/pnas.1616413114
7) Miyagi et al., Nature Nanotechnology 2016, 11: 783-790
8) Takahashi et al., Small, 2016, 12(44):6106-6113
9) Rangl et al., Nature Communications, 2016, 7: doi:10.1038/ncomms12789
10) Ruan et al., in preparation