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Liposomes, Exosomes, and Virosomes: From Modeling Complex
Membrane Processes to Medical Diagnostics and Drug Delivery
Poster Abstracts
100
18-POS
Board 9
Investigation of the Use of Exosomes as Drug Delivery Systems
Ellie Barlow Myers, Salomé Guillaumin, Jean-Marie Devoisselle,
Joel Chopineau
, Marie
Morille.
n/a, , France.
Exosomes, as natural vectors of biomolecules, has been described as interesting drug delivery
systems for mainly nucleic acids, but also lipids or proteins. We propose to use pharmaceutical
and chemical methods (post-insertion, click chemistry,…) to allow drug loading as well as
surface modification to increase the pharmacokinetics behavior and targeting efficiency with
“easy to handle” processes which should facilitate the development of new exosome-based
therapeutics. The efficient exosome-mediated delivery in vivo requires targeting vesicles for
uptake by specific recipient cells, as the half life of non targeted exosomes was close to 2min
after systemic administration in mice (Morishita et al., 2015; Smyth et al., 2015; Wiklander et
al., 2015). As a supplementary hurdle, frequently encountered targeting process used to modify
exosomes, require transfection of cells and lead to low production yield (Alvarez-Erviti et al.,
2011; Hung et al., 2015). In this context, we choose to modify human mesenchymal stem cells
derived exosomes in a post-production manner, by various physico-chemical methods. We are
currently working on the surface modification of exosomes (PEGylation, ligand attachment).
These modifications are assessed by physico-chemical characterization (size, surface charge),
and quantification of the grafted molecules (PEG quantification (KI/I2), ELISA,…) at the
exosome surface. In parallel, the loading ability is evaluated for different kind of molecules
(chemicals, protein, nucleic acids), and different process. The efficiency of surface modification
will be evaluated in vitro in regards to interaction with plasma proteins as well as with immune
cells (THP1 monocytes) thanks to a fluorescent tracking (FACS, confocal microscopy). The
interaction with cells which produced exosomes and other cell lineage (tumoral cell line) will be
evaluated.