115 / 120
Liposomes, Exosomes, and Virosomes: From Modeling Complex
Membrane Processes to Medical Diagnostics and Drug Delivery
Poster Abstracts
110
48-POS
Board 24
Polymer-Supported Lipid Bilayers Derived from Native Cell Membranes as Platforms for
Studying Biological Nanoparticle-Cell Interactions
Hudson Pace
, Nadia Peerboom, Eneas Schmidt, Virginia Claudio, Marta Bally, Fredrik Höök.
Chalmers University of Technology, Göteborg, Sweden.
The ability to produce polymer-supported lipid bilayers (pSLBs) that contain native membrane
components which retain transmembrane protein mobility and activity offers a new paradigm in
the study of nanoparticle-cell surface interactions. These pSLBs are created using native cell
membrane vesicles (NMVs) (i.e., plasma membrane preparations or ghost cells) which allows
these biomimetic surfaces to display the complex composition of the plasma membrane in a
format amenable to a wide range of surface-sensitive analytical techniques. Characterization of
the mobility and functionality of membrane proteins contained within these pSLBs will be
discussed. Additionally, compositional and physical characterization of the NMVs will be
presented.
The utility of this platform will be presented in the context of two systems: 1) the study of
Herpes Simplex Virus (HSV) interactions with the pSLBs derived from monkey kidney (VERO)
cells and 2) the study of exosomes isolated from glioma cell cultures and their interactions with
pSLBs derived from endothelial cells. The HSV/VERO system was used in investigating the
potency of viral-binding inhibitors, while the exosome/endothelial system was used to
investigate the role of exosomes in angiogenesis. Total Internal Reflection Microscopy (TIRFM)
in combination with single-particle tracking of fluorescently labeled biological nanoparticles
provided the ability to investigate single-particle binding kinetics in both systems. These systems
illustrate the potential of single-particle tracking on NMV-derived pSLBs for the comprehensive
investigation of the interaction characteristics of individual biological nanoparticles with native
cell membranes.