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Liposomes, Exosomes, and Virosomes: From Modeling Complex
Membrane Processes to Medical Diagnostics and Drug Delivery
Thursday Speaker Abstracts
44
Extracellular Vesicles for Trans-Blood Brain Barrier Drug Delivery
Matthew Wood
.
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United
Kingdom.
Extracellular vesicles (EVs) are natural nanovesicles whose function is the intercellular transport
of protein and RNA components. Given the urgent requirement for more effective application
and delivery of biotherapeutic agents in the treatment of neurological disorders we have sought
to exploit the natural properties of EVs and EV-based technologies for this purpose. We have
therefore begun to develop a range of EV-based platform technologies including; EV display of
brain-targeting for trans-blood brain barrier delivery; EV delivery nucleic acid-based therapeutic
agents; EV display of biotherapeutic agents; novel methods for EV isolation. EVs comprising
these drug cargoes or for biotherapeutic display have been evaluated in vitro and in vivo, to
evaluate biodistribution, brain penetration and efficacy in animal disease models. In particular
we have begun to study gene silencing applications for neurodegenerative disease and methods
for modulating neuroinflammation. Future prospects for developing and exploiting EV-based
technologies for regenerative medicine applications will be discussed.
1: Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJ. Delivery of siRNA
to the mouse brain by systemic injection of targeted exosomes. Nature Biotechnology 2011
Apr;29(4):341-
2: El-Andaloussi S, Lee Y, Lakhal-Littleton S, Li J, Seow Y, Gardiner C,
Alvarez-Erviti L, Sargent IL, Wood MJ. Exosome-mediated delivery of siRNA in
vitro and in vivo. Nature Protocols 2012 Dec;7(12):2112-26
3: EL Andaloussi S, Mäger I, Breakefield XO, Wood MJ. Extracellular vesicles:
biology and emerging therapeutic opportunities. Nature Reviews Drug Discovery 2013
May;12(5):347-57