6
Issues
•
The FC assay may not give an accurate
estimation of phenolic contents of plants,
extracts or supplements which also contain
th FC ti
b t
o er ‐reac ve su s ances
•
The FC assay is a nonspecific antioxidant assay
•
Many possible interferences including ascorbic
acid
Waterhouse, A.L., Determination of Total Phenolics, in Current Protocols in Food Analytical Chemistry, I1.1.1‐I1.1.8,
Wrolstad,R.E., Wiley, 2001, or Singleton, V. L.; Orthofer, R.; Lamuela‐Raventos, R. M. Analysis of total phenols and
other oxidation substrates and antioxidantsby means of Folin‐CiocalteuReagent. Methods in Enzymology 1999, 299, 152‐178.
