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Liton Roy PhD
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Developed LCMS methods for quantitative and qualitative analysis of small molecules.
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Developed and validated peptide fingerprinting, quantitative FTNIR, and quantitative SDS-PAGE
methodologies.
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Served as the subject matter expert for new product development and participated in related
cross-functional teams. Driven analytical projects to support pre-clinical studies.
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Resolved key method issues in QC by implementing advanced and reliable analytical methods
resulting in 225,000 USD savings in one fiscal year.
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Initiated third party scientific collaboration to address existing QC problems.
University of Vermont, Burlington, VT (Graduate Teaching Assistant), 2006-2011
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Developed a new high throughput screening approach to select, identify, and characterize highly
stable globular proteins.
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Developed FPLC, HPLC, ESI-MS methodologies for the analysis of peptide libraries and bio-
mimetic complexes.
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Utilized nanospray mass spectrometry technique for ligand exchange kinetic study.
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Designed metal directed coiled coil systems and analyzed electrostatic determinants of stability.
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Routinely utilized GC, NMR, Mass spectrometry, and FTIR for small molecule characterizations.
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Routinely utilized solid and solution phase peptide synthesis to produce peptide library (mixtures
and discrete).
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Led interdisciplinary research projects to completion.
Michigan State University, East Lansing, MI (Research and Teaching Assistant) 2003-2005
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Developed HPLC and FPLC methodologies for enzymatic assays and protein purification.
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Detailed mechanism of radical generation of SAM enzymes using ENDOR and EPR
spectroscopies.
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Synthesized isotopically labeled AdoMet synthetase by recombinant expression techniques.
Bose Research Institute, Calcutta, India (Summer Intern) Summer 2002
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Characterized active sites of TIM barrel proteins using Rasmol, Protein Blast, and Homology
screening.
Technical Proficiencies and Research Skills
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Over eight years of experience in LCMS (Agilent 6460 QQQ, ABI 4000 QTrap) and MALDI-TOF
(Voyager DE-PRO). Skilled in ESI, APCI, Nanospray, and Direct infusion applications. Used H/D
exchange analysis of small molecules. Skilled in bottom up mass spectrometric analysis of
peptide libraries. Experienced with Masshunter and Analyst software.
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Over ten years of experience in HPLC (RP, SEC, HIC, IEX) method development for small
molecule quantitations, protein/peptide purifications, and separations. Experienced with
Chemstation and Empower software. Executed HPLC/UPLC method transfer. Developed and
validated assay, related substance, and stability indicating methods using DAD, MWD, FLD, and
RI detectors.
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Three years of experience in developing and validating Type I, II, III dissolution methods for
tablets and softgels.
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Expertise in GC-FID analysis of fatty acids and small molecules. Developed and validated GC-
FID and GC-Head space methods under cGMP compliance.
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Over ten years of experience in methodologies of UV/VIS, Fluorescence, CD, FTIR, and NMR
spectroscopies. Experienced with Antaris FTNIR.