© 2012 AOAC INTERNATIONAL
12
12
12
p
i
12
1
2
(
2
) At each working standard concentration, the peak areas of
standards injected at the beginning and end of a set of samples
should not increase or decrease by more than 10%.
(
e
)
Calculation of vitamin B
concentrations in samples.—
The
vitamin B
concentration in each injected sample preparation is
extrapolated from the vitamin B
The concentration of vitamin B
standard curve prepared above.
in each product can then be
12
calculated.
C = C × D
ss × D
V
p
i
1
2
where C
= product concentration in
g/kg; C
= vitamin B
concentration of the injected sample preparation extrapolated from
standard curve in
g/L; D = volume of the first dilution in mL
(100 mL); ss = sample size in g; D = volume of the second (final)
dilution in mL; V = volume of filtrate loaded onto the cartridge in
mL.
For each set of samples, the control result must be within 3
standard deviations of the control mean.
G. Validation Data
Note: SLV and MLT data from the ERP checklist will be added to this section. Data tables will be added by September 19.
References:
J. AOAC Int
.
95
, 313(2012); AOAC SMPR 2011.005
J. AOAC Int
.
95
, 293(2012)
