Engineering Approaches to Biomolecular Motors

Engineering Approaches to Biomolecular Motors: From in vitro to in vivo Poster Abstracts

9-POS

Board 9

Bio-functionalized Vesicles for the Measurement of Biological Activities and their

Automated Generation

Huisoo Jang, Sunhee Yoon, Sun Min Kim.

Tae-Joon Jeon

Inha University, Incheon, South Korea.

We have functionalized polymeric vesicles with antibodies to measure their activities to the

target molecules. Polymeric vesicles are made of polydiacetylene that has chromatic transition

from blue to red upon external stimuli, such as pH, heat, and perturbation of its structure. These

vesicles are further encapsulated within hydrogel matrix to achieve more enhanced biological

activities. This process is fully automated using a microfluidic device. The target molecule is

phosphinothricin acetyltransferase (PAT) that is one of most abundant protein in genetically

modified organisms (GMOs). To recognize PAT, anti-PAT antibody was conjugated onto the

surface of the PDA vesicles using EDC/NHS coupled reaction. PDA vesicles were encapsulated

into the poly(ethylene) glycol diacrylate (PEG-DA) hydrogel at high concentration to achieve

amplified the signal of color. Moreover, we generated a bead type hydrogel using a microfluidic

device in an automated manner. With our system, we were successfully able to detect PAT

protein in a very low concentration with amplified singals.