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Multi Laboratory Test – AOAC First Action Method 2012.22 Ascorbic Acid (Vitamin C) in Infant Formula and
Adult/Pediatric Nutritionals.
RDLS-RD150006
E. Campos Giménez (NRC, Lausanne)
Page 18
CONFIDENTIAL
The contents of this document constitute a trade secret and the intellectual property rights thereto belong to Nestlé S. A., Vevey, Switzerland.
This document may not be reproduced or disclosed to third parties without prior authorisation.
Annex B: Method,
Continued
I.
Example chromatograms
Infant formula powders/Certified reference material
Nutritional products
References
(1)
Fontannaz, P., Kilinc, T., and Heudi, O. (2006)
Food Chemistry.
94,
626-631.
(2)
Campos-Giménez, E., Fontannaz, P., Redeuil, K., Kilinc, T. (2013)
Journal of AOAC
International
,
96
, 1065-1067.
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Standard solution Ascorbic acid 2 µg/mL
Infant FormulaPowder Milk Based
Infant FormulaPowder Soy Based
SRM 1849a
Ascorbic
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Standard solutionAscorbic acid7.5 µg/mL
Infant Elemental Powder
Adult Nutritional RTF HighFat
AOAC OMB Meeting Book
352