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26
Table 2016.2B (cont’d.)
: Comparative Results for the Detection of
Listeria monocytogenes
in 25 g Raw Chicken Breast Fillet Test Portions by the 3M
™
MDA 2 -
1
Listeria monocytogenes
Method vs. USDA/FSIS MLG Chapter 8.09 in a Collaborative Study (Low Inoculum Level)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate presumptive
(CP)
Candidate confirmed (CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Chicken
Breast
Fillet
1
12
7
0.58
12
7
0.58
12
7
0.58
12
7
0.58
0.00
0.00
2
12
9
0.75
12
9
0.75
12
9
0.75
12
7
0.58
0.17
0.00
3
12
10
0.83
12
10
0.83
12
10
0.83
12
5
0.42
0.41
0.00
4
12
9
0.75
12
9
0.75
12
9
0.75
12
4
0.33
0.42
0.00
5
12
8
0.66
12
8
0.66
12
8
0.66
12
5
0.42
0.22
0.00
6
12
9
0.75
12
9
0.75
12
9
0.75
12
6
0.50
0.15
0.08
7
12
7
0.58
12
6
0.50
12
6
0.50
12
6
0.50
0.00
0.16
8
12
7
0.58
12
7
0.58
12
7
0.58
12
7
0.58
0.00
0.00
9
12
8
0.66
12
9
0.75
12
8
0.66
12
7
0.58
0.08
-0.08
10
b
12
6
0.50
12
8
0.66
12
6
0.50
12
5
0.42
0.08
-0.16
MPN/
Test
Portion
0.66
0.51
0.83
11
a
NA NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
12
12
7
0.58
12
7
0.58
12
7
0.58
12
4
0.42
0.16
0.00
13
12
5
0.42
12
5
0.42
12
5
0.42
12
6
0.50
-0.08
0.00
Estimate
All
132
86
0.65
132
86
0.65
132
85
0.64
132
64
0.48
0.16
0.00
LCL
0.57
0.57
0.56
0.40
0.04
-0.12
UCL
0.73
0.73
0.73
0.57
0.28
0.12
s
r
c
0.48
0.48
0.48
0.51
LCL
0.43
0.43
0.43
0.46
UCL
0.52
0.52
0.52
0.52
s
L
d
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.17
0.18
0.18
0.14
s
R
e
0.48
0.48
0.49
0.51
UCL
0.43
0.43
0.43
0.46
LCL
0.52
0.52
0.52
0.52
P
T
f
0.7057
0.5632
0.6228
0.9192
a
N/A – Laboratory did not participate or submit data for this matrix;
b
Results were not used in statistical analysis due to deviation from testing protocol.
3
c
Repeatability Standard Deviation,
d
Among-Laboratory Standard Deviation,
e
Reproducibility Standard Deviation,
f
P
T
Value = Homogeneity test of laboratory PODs
4
LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
5
6
7
8
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only