529 / 596
9
(a)
Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
Specific Instructions for Validated Methods
AOAC® Performance Tested Method
sm
# 081501
In AOAC PTM studies, the 3M Molecular Detection Assay 2 –
Listeria monocytogenes
was found
to be an effective method for the detection of
Listeria monocytogenes.
The matrices tested in the
study are shown in Table 3. The limit of detection of the 3M Molecular Detection Assay 2 –
Listeria
monocytogenes
method is 1-5 colony forming units per validated test portion size (in Table 3).
Table 3.
Enrichment protocols using Demi-Fraser Broth at 37 ± 1 °C
according to AOAC Performance Tested
SM
Certificate #081501.
Sample Matrix
Sample Size
Enrichment
Broth Volume
(mL)
Enrichment
Time (hr)
Beef hot dogs, Queso
Fresco, Vanilla Ice
Cream, 4% Milk Fat
Cottage Cheese, 3%
chocolate whole milk,
romaine lettuce, bagged
raw spinach, cold
smoked salmon
25 g
225
24-30
Raw chicken
25 g
475
28-32
Deli turkey
125 g
1125
24-30
Cantaloupe
(a)
Whole melon
Enough volume
to allow melon
to float
26-30
e
s
:
Stainless
1 sponge
225
24-30
Fraser Broth