56-57 / 596
3M MDA 2
Listeria
species and
L. monocytogenes
Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
Appendix 8.3b – Study Flow Diagram: Raw Chicken Breast
1
2
3
Raw chicken breast- 25g
3MMDA 2-
Listeria
&
3MMDA 2-
L.
monocytogenes
25g + 475 mL
prewarmed (37±1C
°
)
Demi Fraser (no FAC),
28-32 hr at 37±1C
°
Presumptive Result
Using enriched sample,
run 3M MDA
Listeria
monocytogenes
test kit
MDA Confirmation
Swab and streak to
MOX, 24-28 hr hr at
35±2C
°
Streak suspect colonies
to TSA, 24 hr at 37±1
°
C
ID at least one colony
using VITEK 2 GP or API
Listeria
MDA Confirmation
Transfer 0.1 mL to
Fraser broth, 24-28 hr hr
at 35±2C
°
Swab and streak to
MOX, 24-28 hr hr at
35±2C
°
Streak suspect colonies
to TSA, 24 hr at 37±1
°
C
USDA/FSIS MLG 8.09
25g + 225 mL UVM
20-26 hr at 30±2C
°
Transfer 0.1 mL to
Fraser broth, 24-28 hr hr
at 35±2C
°
Swab and streak to
MOX, 24-28 hr hr at
35±2C
°
Streak suspect colonies
to TSA, 24 hr at 37±1
°
C
ID at least one colony
using VITEK 2 GP or API
Listeria
Swab and streak to
MOX, 24-28 hr hr at
35±2C
°
Streak suspect colonies
to TSA, 24 hr at 37±1
°
C
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only