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Bystander and non-target irradiation effects on normal and
radiosensitive lymphocytes
• Used normal and radiosensitive cell lines
– Irradiated in- and out of field 6MV
– +/- Media from one transferred to others
•
Out-of-field radiation induces g-H2AX expression in radiosensitive cells
•
Out of field radiation decreases growth of radiosensitive cells
•
Out of field radiation and addition of irradiated cell conditioned media differentially
affects cell line responses
•
Treatment of normal responding cells with conditioned media from cells of different
radiosensitivities varies their cytokine gene expression pattern
•
Out of field radiation increases DNA damage in primary human peripheral blood
mononuclear cells
• Conclude out of field radiation induces early DNA damage in radiosensitive cell lines
and primary blood cells and reduces cell proliferation over 5 days.
– Implications for radiosensitive patients
• Important to determine out of field dose accurately and investigate effects.
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J. Bryant, L. Shields, C. Hynes, M. Taylor, O. Howe, BMcClean, F. Lyng
Submitted June 2018