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Biophysics in the Understanding, Diagnosis, and Treatment of Infectious Diseases Poster Abstracts
71
29-POS
Board 29
The Interaction of RNA Helicase DDX3 with HIV-1 Rev-CRM1-RanGTP Complex during
the HIV Replication Cycle
Hanif Mahboobi, Alex Javanpour,
Mohammad Mofrad
.
University of California, Berkeley, CA, USA.
Nucleocytoplasmic macromolecullar shuttling is regulated by the nuclear pore complex (NPC),
which acts as a highly selective channel perforating the nuclear envelope in eukaryotic cells. The
human immunodeficiency virus (HIV) exploits the nucleocytoplasmic pathway to export its
RNA transcripts across the NPC to the cytoplasm. Despite extensive study on the HIV life cycle
and the many drugs developed to target this cycle, no current drugs have been successful in
targeting the critical process of viral nuclear export, even though HIV’s reliance on a single host
protein, CRM1, to export its unspliced and partially spliced RNA transcripts makes it a tempting
target. Due to recent findings implicating a DEAD-box helicase, DDX3, in HIV replication and a
member of the export complex, it has become an appealing target for anti-HIV drug inhibition.
We present a hybrid computational protocol to analyze protein-protein interactions in the HIV
mRNA export cycle. Using this method, we highlight some of the most likely binding modes and
interfacial residues between DDX3 and CRM1 both in the absence and presence of RanGTP.
This work shows that although DDX3 can bind to free CRM1, addition of RanGTP leads to more
concentrated distribution of binding modes and stronger binding between CRM1 and RanGTP.