Single-Cell Biophysics: Measurement, Modulation, and Modeling

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Single-Cell Biophysics: Measurement, Modulation, and Modeling Poster Abstracts
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101-POS Board 51
Stretching Single Native Chromain Fiber in Nanofluidic Channel for Detection of
Epigenetics Marks
Jia-Wei Yeh2,1, Kylan Szeto2.
1Academia Sinica, Taipei, Taiwan, 2Cornell University, Ithaca, NY, USA.
Stretching single chromosomal DNA fibers in nanofluidic devices has become a valuable tool for
studying the genome and epigenetics. Although nanofluidic technology has been extensively
used in single molecular DNA analysis applications, compared to bare DNA, much less work has
been done to elongate and analyze native eukaryotic chromatin fibers. Here, we provide a
method for stretching and imaging individual chromatin fibers. A micro- and nanofluidic device
was used to electrophoretically stretch and image single native chromatin fibers attached to
microspheres held at the entrance of a nanoslit. Chromatin fragments extracted from human
cancer cells (HeLa cells) were stretched and held in nanoslits, and the histone modification
H3k79me2 was optically detected by fluorescence microscopy.