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Single-Cell Biophysics: Measurement, Modulation, and Modeling
Monday Speaker Abstracts
30
Microfluidic Single Cell Sequencing
Yanyi Huang
.
Peking University, Beijing, China.
Biosystems are intrinsically heterogeneous. Conventional ensemble measurements heavily rely
on ‘average’ values from multiple experimental replicates to study the quantitative difference
between samples, or between conditions. However, the heterogeneity at the cellular level make
such strategy impossible in many cases. Next generation sequencing technologies allow us to
obtain large and informative data-set with relatively low cost and high throughput, and become
the key driving force to facilitate our understanding of complex biosystems. However, prevalent
methods are still problematic when handle highly heterogeneous samples because of the mix of
real biological variations and technical variations or noises. I am going to present recent
technology developments in my group, mostly the combination usage of microscopic imaging
with microfluidics, to facilitate the approaches using next generation sequencing tools to analyze
small number of cells or even single cells.