Biophysical Society Thematic Meeting - June 28-July 1, 2015

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New Biological Frontiers Illuminated by Molecular Sensors and Actuators Poster Abstracts
39-POS Board 39
Withdrawn
Kalpana Settu1, Ching-Jung Chen2, Jen-Tsai Liu2, Jang-Zern Tsai1.
1National Central University, Taoyuan, Taiwan, 2University of Chinese Academy of Sciences,
Beijing, China.
40-POS Board 40
A FRET-based Calcium Biosensor based on Calmodulin (CaM) and Its Newly Identified
Binding Peptide from c-FLIPL
Wang Sheng.
Peking university, Beijing, China.
Genetically encoded calcium biosensors are valuable tools in cell biology and neuroscience. A
FRET-based calcium biosensor Cameleon has received a great deal of optimization since it was
released in 1997. Despite all the extensive improvements, the sensing and functional domains of
Cameleon were solely based on engineering of the fusion protein CaM and its binding peptide
from myosin light-chain kinase (M13). Whether there exist other feasible CaM-binding peptides
that can replace M13 in sensor construction has not been explored. Here, we report a FRET-
based calcium biosensor comprising CaM and its newly identified binding peptide from c-
FLIPL. We tested the performance of this new CaM-binding peptide from c-FLIPL in
construction of functional FRET-based biosensors. We also performed preliminary optimization
and application in imaging of Ca2+ dynamics in living cells.