115 / 154
Study Information,
Continued
Statistical
evaluation
After data collection, outliers were detected using Cochran and Grubbs tests. The
number and coded identity of statistical outlier laboratories are included in this
report.
Average chloride concentrations, standard deviations of repeatability (S
r
) and
relative standard deviations of repeatability (RSD
r
) were estimated from blind
duplicates in MLT samples. The duplicates were assigned to be analyzed on the
same day.
Standard deviations of reproducibility (S
R
), relative standard deviations of
reproducibility (RSD
R
), and HORRAT values (RSD
R
/predicted RSD
R
) were also
estimated.
Details on statistical analysis can be found in the “Appendix D: Guidelines for
Collaborative Study Procedures To Validate Characteristics of a Method of
Analysis” of the Official Methods of Analysis of AOAC INTERNATIONAL, 18th
Edition (2005).
Materials, Method and Laboratories
Matrices
The study took place using SPIFAN matrices, which represent most of the
products in the scope of the project (Infant Formula and Adult Nutritionals
made from any combination of milk, soy, rice, whey, hydrolyzed protein, starch
and amino acids, with and without intact protein). In addition, IDF matrixes
were added to this MLT in planning to be able to expand the scope to include
dairy. All samples were codified.
The complete list of samples can be found in Annex A.
Homogeneity
Tests
SPIFAN matrixes:
Homogeneity tests were performed by analyzing several compounds (i.e.
calcium, manganese, zinc, pyridoxine and tocopheryl acetate). The testing
was performed by Covance Laboratories. The report is available upon request
at AOAC.
IDF matrixes:
Homogeneity tests were performed by analyzing several compounds (i.e.
calcium, manganese, zinc, fat and chloride). The testing was performed by
MUVA kempten. The report is available upon request from MUVA.
Method
The full method description is given in Annex B.
Participating
laboratories
Twenty three laboratories tentatively agreed to participate in the study. The
complete list can be found in Annex C. Random codes were attributed to the
laboratories for identification.
2016.03 (APRIL 2017) - MTE-01/03
MLT REPORT
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