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PROTOCOL
•
Elution
•
Concentration
CEN/TC275/WG6/TAG4
ISO/TS 15216
PART 1 & 2
Virus : detection method?
QUANTITY
PURIFIED
VIRAL RNA
From a know volume of
concentrate/extraction by standard
method
100
cm
2
25g
2g
1L
5
Qualitative
Quantitative
ANALYSIS
2 PARTS
Real-time (TaqMan) RT-PCR
Determination
Virus : detection method?
CEN/TC275/WG6/TAG4
ISO/TS 15216
Part 1 & 2
PRETREATMENT & EXTRACTION OF
NUCLEIC ACIDS
SWABBING FOLLOWED BY ELUTION
100c
m
2
25g
ELUTION WITH AGITATION & PRECIPITATION
WITH PEG / NaCl
6
2g
1L
DIGESTIVE TISSUE PRETREATMENT BY
CRUSHING AND PROTEINASE K
ADSORPTION & ELUTION ON LOADED MEMBRANES &
CONCENTRATION / ULTRAFILTRATION