17 / 20
Std
L-Theanine
concentration
L-Norleucine
concentration
Relative back-calculated errors, %
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
1
100 ug/mL
10 ug/mL
0.37
0.51
-0.31
0.17
0.69
0.29
2
80 ug/mL
10 ug/mL
0.02
1.21
0.28
-0.11
-0.05
-0.88
3
60 ug/mL
10 ug/mL
0.09
0.26
0.22
0.82
-0.06
0.36
4
50 ug/mL
10 ug/mL
0.14
-0.41
0.88
0.01
-0.55
1.08
5
40 ug/mL
10 ug/mL
-0.78
-1.01
-0.14
-0.73
0.02
0.24
6
25 ug/mL
10 ug/mL
-0.67
-0.73
-0.49
-0.49
0.14
-0.49
7
20 ug/mL
10 ug/mL
-1.35
-2.13
-0.63
0.16
-0.71
-1.45
8
10 ug/mL
10 ug/mL
1.35
-1.99
0.68
-0.69
0.02
-0.25
9
7.48 ug/mL
10 ug/mL
1.05
-1.91
-1.03
-1.36
0.01
-2.03
10
5 ug/mL
10 ug/mL
-0.02
-3.33
-1.65
-0.30
-0.48
1.69
11
2 ug/mL
10 ug/mL
3.74
3.49
-3.55
-1.75
0.82
2.13
12
1 ug/mL
10 ug/mL
4.19
-0.79
1.09
-0.17
-0.33
4.24
13
0.5 ug/mL
10 ug/mL
1.70
6.82
14.9
4.43
0.88
-4.95
Table 8. Ruggedness Trial Experimental Design
Factor
Value 1
Value 2
Formulation of Ninhydrin Reagent
T100, 1-part Ninhydrin Reagent (A)
T200, 2-part Ninhydrin Reagent (a)
HPLC Flow Rate
0.35 mL/min (B)
0.38 mL/min (b)
Extraction Volume
25 mL (C)
10 mL (c)
Analyst
Analyst 1 (D)
Analyst 2 (d)
Extraction time
2h (E)
1.5 h (e)
Extraction Solution Li220
Lot 1 (F)
Lot 2 (f)
Reactor Temperature
130
o
C (G)
125
o
C (g)
Experiment
Number
Combination of Factors
1
ABCDEFG
2
ABcDefg
3
AbCdEfg
4
AbcdeFG
5
aBCdeFG
6
aBcdEfG
7
abCDefG
8
abcDEFg
Table 9. Results of the Ruggedness Trial
The Effect of Changing Factors Calculated as Described (20)
√
*SD = 0.0639
A-a
0.0467 <
√2
*SD
B-b
0.0002 < √2 *SD