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3/10/2017
4
Background
Free alpha amino acids and related compounds
β‐alanine
Alanine
Arginine
Asparagine
Aspartic Acid Cysteine
Cystine
Glutamic Acid
Glutamine Glycine
Histidine
Hydroxyproline
Isoleucine Leucine
Lysine
Methionine
Phenylalanine Proline
Serine
Taurine
Threonine Tryptophan Tyrosine
Valine
SMPR Key Points
Method Performance Requirements
Parameters
Acceptable Criteria
Analytical Range (%)
0.04 ‐ 100
LOQ (%)
≤0.04
Recommended
LOD (%)
≤0.01
For individual free amino acid components measured.
Ranges (%)
0.04 ‐10
> 10
Recovery (%)
90 ‐ 107
98 – 102
% RSD
r
≤ 5
≤ 3
% RSD
R
≤ 8
≤4
For individual free amino acid components measured.