AOAC SPIFAN Review Team Meeting Book-July 22, 2015

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Table 1. Precision and Spike recovery results for beta-carotene
Beta-carotene Performance
Analytical range 10 µg/kg – 2.8 mg/kg
Limit of quantitation (LOQ) ≤ 10 µg/kg
Linearity (10 points) 0.01 – 10 mg/L: r2 = 0.9993
Repeatability
(n = 6-fold, RSDr )
9.2 µg/kg (SM) 4.5 %
110 µg/kg (SM + spike) 0.9 %
520 µg/kg (IF) 4.2 %
2820 µg/kg (IF + spike) 2.9 %
Reproducibility
(n = 6 duplicates, RSD R)
9.2 µg/kg (SM)
110 µg/kg (SM + spike)
520 µg/kg (IF)
2820 µg/kg (IF + spike)
Recovery
(n = 6)
110 µg/kg (SM + spike) 97 %
2820 µg/kg (IF + spike) 109 %
SM = skimmed milk (liquid). IF = infant formula (powder).
Results are based on the total of cis and trans isomers.
Table 2. Precision and Spike recovery results for lutein
Lutein Performance
Analytical range 71 µg/kg – 4.6 mg/kg
Limit of quantitation (LOQ) ≤ 71 µg/kg
Linearity (10 points) 0.02 – 3.3 mg/L: r2 = 0.9999
Repeatability
(n = 6-fold, RSDr )
not detectable (SM) -
65 µg/kg (SM + spike) 2.4 %
1210 µg/kg (IF) 2.1 %
5020 µg/kg (IF + spike) 2.4 %
Reproducibility
(n = 6 duplicates, RSD R)
not detectable (SM)
65 µg/kg (SM + spike)
1210 µg/kg (IF)
5020 µg/kg (IF + spike)
Recovery
(n = 6)
65 µg/kg (SM + spike) 98 %
5020 µg/kg (IF + spike) 101 %
SM = skimmed milk (liquid). IF = infant formula (powder).
Results are based on the total of cis and trans isomers.
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