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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method:
BVit-03 #1
Title:
Determination of B-Vitamins in Infant Formula and Adult/Pediatric Formulas
Author:
TUV/SUD
Summary of Method:
Samples are extracted with water in presence of ascorbic acid and adjusted to pH 4.8 with
ammonia solution to precipitate proteins. Extract is filtered and subjected to LC-MSMS. Results are
quantified against certified reference materials by external standard method.
Method Scope/Applicability:
Infant Formula and Adult/Pediatric Formulas
General comments about the method:
Method describes the analysis of a number of B-group vitamins using LC-MS/MS. Only free supplemental
vitamin forms are measured and the method does not include measurement of phosphorylated forms
specified in the SMPR applicability.
Method Clarity:
Method clarity and detail sufficient, however equations detailing the calculation of standard concentrations
needed.
Method Safety Concerns:
None
Pros/Strengths:
Relatively simple straightforward analysis.
Cons/Weaknesses
Quantitation is by external standardisation only which can be problematic in LC-MS assays.
Supporting Data
Data from the SPIFAN kit has not been reported. Of the analytes under consideration as part of the B-
group SMPRs, repeatability is within the SMPR limits for riboflavin and pyridoxine only. Recovery is within
SMPR limits for riboflavin and niacinamide.
The only sample reported is the NIST 1849a and unfortunately it is unclear from the report how the results
obtained compare to certified value.
Performance Characteristics:
BVit-03 Review Forms