Table of Contents Table of Contents
Previous Page  53 / 159 Next Page
Information
Show Menu
Previous Page 53 / 159 Next Page
Page Background

FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

1

Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method:

Carot-01

#2

Title:

Determination of Carotenoids in Infant Formula and Adult/Pediatric Nutritional Formula using High

Performance Liquid Chromatography with Photo Diode Array Detection

Author:

Martijn Vermeulen, Ausnutria

Summary of Method:

Carotenoid esters are saponified using potassium hydroxide in ethanol/water in the presence of sodium

ascorbate, sodium sulfide and glycerol to prevent oxidation. Dimethyltocol (DMT) is added as an internal

standard and carotenoids are extracted using diisopropyl ether. The extract is subsequently washed and

concentrated. Carotenoids are determined by RPLC-UV at 450 nm and DMT is measured at 292 nm.

Method Scope/Applicability:

Infant Formula and Adult/Pediatric Formulas

General comments about the method:

Method appears to measure carotenoid forms specified in the SMPR, however, this is not explicitly stated.

Using this chromatography, it is not possible to get sufficient resolution to unambiguously integrate the cis-

isomers of lutein and a-carotene which are explicitly to be determined in the applicability statement.

Method Clarity:

Clear directions for operation of the method, instruments. No details about how to make up a-carotene,

lutein, lycopene standards, or mixed calibration standards. Clear equations describing how the standards

and samples are quantitated is required.

Method Safety Concerns:

None other than standard laboratory safety

Pros/Strengths:

Very simple straightforward analysis

Cons/Weaknesses

Supporting Data

SPIFAN kit was used in the validation of the method.

Results reported on dry weight basis, making interpretation against SMPR difficult.

Performance Characteristics:

Carot-01 Review Forms