101 / 128
GENERAL ANALYTICAL NEEDS
•
Method should
–
Quantify collagen Type I, II & III in raw materials and finished product
matrices.
–
Specific enough to individually separate and quantify collagen type I, II
& III if blended together.
–
Identify the species of the collagen whether it is derived from marine,
chicken, bovine or porcine sources.
–
Identify the adulterant(s) as other protein in the collagen samples.
GENERAL OVERVIEW OF
THE EXISTING METHODS & TECHNIQUES
•Nitrogen based protein analysis methods to calculate the collagen
content
(unspecific)
•Other total protein analysis methods
(unspecific)
•Western blot using specific collagen antibodies.
(Not effective in
hydrolyzed collagen samples)
•ELISA for specific type of collagen.
(Not effective in hydrolyzed
collagen samples)
•Hydroxyproline based collagen assay method.
(Not type or species
specific)