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Submission Date
2016-07-30 04:41:22
Name
Darryl Sullivan
Organization
Covance Laboratories
Title of Method
Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts,
and Dietary Supplements by HPLC-UV: Single Laboratory Validation.
AOAC Candidate Method
Number (e.g. ALN-01)
TEA-02
Applicable SMPR
2015.014
Summary:
This method can quantify seven catechins and and caffeine in green tea raw materials,
and powdered extracts and dietary supplements containing green tea extract. The
method utilizes reverse phase HPLC with a phenyl-based stationary phase and and
gradient elution. Detection is accomplished using UV absorption.
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
This method is capable of measuring catechins and caffeine, but has not been
demonstrated to be capable of determining methyl xanthines, theaflavins, or theanine.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
The method appears to meet the SMPR for catechins.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
yes
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
yes
AOAC SPDS ERP - Set 3 Review Form 2