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141

Biophysical Society 59

th

Annual Meeting, Baltimore, Maryland

T

U

E

S

D

A

Y

Optical Microscopy and Super-Resolution

Imaging II (Boards B529-B558)

2392-P

os

B

oard

B529

international

travel awardee

3 COLOR - 3 DIMENSIONAL STED NANOSCOPY.

Chiara Peres

,

Michele Oneto, Francesca D’Autilia, Silvia Galiani, Luca Lanzano’,

Giuseppe Vicidomini, Alberto Diaspro, Paolo Bianchini

2393-P

os

B

oard

B530

EFFICIENT INTEGRATED 3D AND MULTI-COLOR SINGLE

MOLECULE SUPER-RESOLUTION IMAGING. Kenny Chung,

Tobias Hartwich,

David Baddeley

2394-P

os

B

oard

B531

THREE-DIMENSIONAL SUPER-RESOLUTION PROTEIN

LOCALIZATION CORRELATED WITH VITRIFIED CELLULAR

CONTEXT.

Wei Ji

, Bei Liu, Yanhong Xue, Wei Zhao, Tao Xu

2395-P

os

B

oard

B532

3D MICROSCOPY OF ROD-SHAPED BACTERIA REVEALS

ROLES OF MREB IN DIAMETER CONTROL AND CENTER-

LINE CURVATURE.

Benjamin P. Bratton

, Jeffrey P. Nguyen, Nikolay

Ouzounov, Randy M. Morgenstein, Zemer Gitai, Joshua P. Shaevitz

2396-P

os

B

oard

B533

ENABLING SINGLE-MOLECULE DETECTION IN LIVING

CELLS: ULTRA-SENSITIVE MICROSCOPY AND SPECTROSCOPY

IN 3D.

Ankun Dong

, Alexandros Pertsinidis

2397-P

os

B

oard

B534

ANALYSIS OF NANOSCALE PROTEIN CLUSTERING WITH

QUANTITATIVE LOCALIZATION MICROSCOPY.

Philip R. Nicovich

, Thibault Tabarin, Johannes Stiegler, Sophie V.

Pageon, Katharina Gaus

2398-P

os

B

oard

B535

VIDEO-RATE SUPER RESOLUTION MICROSCOPY IN LIVING

CELLS.

Fang Huang

, Caroline E. Laplante, Yu Lin, Thomas D. Pollard,

Joerg Bewersdorf

2399-P

os

B

oard

B536

DUAL-OBJECTIVE POINTILLISM MICROSCOPY SETUP WITH

INTERFEROMETRIC AND ASTIGMATIC DETECTION.

Nora C.

Schmidt

, Jana Hüve, Jürgen Klingauf

2400-P

os

B

oard

B537

HIV GP120 EPITOPE PROFILE ON CELL-BOUND

VIRIONS ASSESSED USING VARIOUS MICROSCOPY

TECHNIQUES.

Meron Mengistu

, George K. Lewis, Anthony L.

DeVico

2401-P

os

B

oard

B538

STRUCTURAL STUDY OF CILIARY TRANSITION ZONE WITH

MULTICOLOR 3D STORM.

Xiaoyu Shi

, Galo Garcia, Jeremy F. Reiter,

Bo Huang

2402-P

os

B

oard

B539

CHARACTERIZATION OF CEACAM1 STRUCTURE AND

DYNAMICS BY CORRELATED HOMO-FRET AND DSTORM

IMAGING.

Amine Driouchi

, Scott D. Gray-Owen, Christopher M. Yip

2403-P

os

B

oard

B540

SUB-DIFFRACTION LIMIT CHARACTERIZATION OF

THE HIGHER-ORDER STRUCTURE AND NANOSCALE

CYTOSKELETAL ARRANGEMENT OF SEPTINS BY

DSTORM.

Adriano Vissa

, Theodore Pham, William S. Trimble, Peter K.

Kim, Christopher M. Yip

2404-P

os

B

oard

B541

DYNAMICS OF THE ACTIN CYTOSKELETON AND PLASMA

MEMBRANE AT THE IMMUNOLOGICAL SYNAPSE REVEALED

USING LIVE-CELL SUPER-RESOLUTION MICROSCOPY.

George W. Ashdown

, Andrew Cope, Paul W. Wiseman, Dylan M. Owen

2405-P

os

B

oard

B542

FULL FIELD NONLINEAR STRUCTURED ILLUMINATION

MICROSCOPY WITH STED. Han Zhang,

Yu Li

, Ming Zhao, Leilei

Peng

2406-P

os

B

oard

B543

REDUCING PHOTOBLEACHING IN STED MICROSCOPY WITH

HIGHER SCANNING SPEED.

Yong Wu

, Xundong Wu, Ligia Toro,

Enrico Stefani

2407-P

os

B

oard

B544

DNA-PAINT AND EXCHANGE-PAINT FOR MULTIPLEXED 3D

SUPER-RESOLUTION MICROSCOPY.

Jesse L. Silverberg

, Ralf

Jungmann, Maier S. Avendano, Johannes B. Woehrstein, Mingjie Dai,

Peng Yin

2408-P

os

B

oard

B545

QUANTITATIVE MULTIPLEXED SUPER-RESOLUTION

NEURONAL SYNAPSE IMAGING USING DNA-PAINT.

Syuan-Ming Guo

, Remi Veneziano, Russell E. McConnell, Sarit Agasti,

Simon Gordonov, Tony Kulesa, Frank B. Gertler, Paul Blainey, Ed

Boyden, Peng Yin, Mark Bathe

2409-P

os

B

oard

B546

SINGLE-MOLECULE DIGITAL IMAGING WITH MOLECULAR

RESOLUTION USING DNA-PAINT.

Mingjie Dai

, Ralf Jungmann,

Peng Yin

2410-P

os

B

oard

B547

CHARACTERIZING FIBROSIS IN MOUSE KIDNEY USING AUTO

FLUORESCENCE FLIM AND SHG IN UUO MODEL.

Moshe Levi

, Evgenia Dobrinskikh, Suman Ranjit, John Montford,

Alexander Dvornikov, Allison Lehman, Seth Furgeson, Raphael

Nemenoff, Enrico Gratton

2411-P

os

B

oard

B548

HIGH SENSITIVITY NDD FLIM AND PATTERN MATCHING

BASED FLUOROPHORE IDENTIFICATION.

Marcelle Koenig

,

Benedikt Kraemer, Volker Buschmann, Matthias Patting, Felix Koberling,

Rainer Erdmann

2412-P

os

B

oard

B549

METABOLIC PROFILING OF THE SKIN TO MONITOR

THE ONSET AND PROGRESSION OF SQUAMOUS CELL

CARCINOMA THROUGH TIME- AND WAVELENGTH-

RESOLVED FLUORESCENCE LIFETIME IMAGING.

Christina R. Miller

, Michael G. Nichols

2413-P

os

B

oard

B550

MAPPING P53 ALTERATIONS IN METABOLISM UPON DNA

DAMAGE USING THE PHASOR/FLIM AND NUMBER &

MOLECULAR BRIGHTNESS ANALYSIS.

Michelle A. Digman

,

Swathi Bagilthaya

2414-P

os

B

oard

B551

international

travel awardee

QUANTITATIVE ANALYSIS OF ANCHORING PROTEINS OF

THE INHIBITORY SYNAPSE THROUGH SINGLE MOLECULE

LOCALIZATION TECHNIQUES.

Francesca Pennacchietti

, Sebastiano

Vascon, Alessio Del Bue, Enrica Maria Petrini, Andrea Barberis, Francesca

Cella Zanacchi, Alberto Diaspro