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Nowadays no standard test…
Buckley et al, Aust NZ J Surg (2015)
IHC/ISH/PCR
PROS
CONS
p16 INK4a immunohistochemistry
routinely used as a cost-effective
surrogate marker
p16 overexpress ion may occur
independently of HPV
DNA in situ hybridization (ISH)
high specificity for detecting active
viruses
less sensitive when there are low viral
copy numbers
RNAscope (ISH)
97% sensi t ivi ty and relat ively
inexpensive
HPV-DNA PCR
experimental settings because of
high sensitivity
it does not identify whether the virus is
transcriptionally active
E6/E7 HPV-mRNA PCR
the gold standard
high costs
The mere detection of HPV-DNA is not
sufficient to establish causality in HNCs!!!
Castellsagué et al, J Natl Cancer Inst (2016)
DIAGNOSIS OF HPV-RELATED LESIONS
mercoledì 29 giugno 16