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Draft, Do Not Distribute
1 Draft EEVs SMPR V4
AOAC SMPR 2015.XXX; Version 5, June 19, 2015
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Method Name:
Identification of Venezuelan Equine Encephalitis Virus (VEEV)
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Approved Body:
AOAC Stakeholder Panel on Agent Detection Assays
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1.
Intended Use
:
Laboratory or field use by Department of Defense trained operators.
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2.
Applicability
:
Identification of VEEV in liquid samples from aerosol collectors.
The
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preferential method would be a field-deployable assay.
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3.
Analytical Technique
:
Molecular methods of detecting target-specific viral component(s).
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4.
Definitions
:
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Acceptable Minimum Identification Level (AMIL)
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The predetermined minimum level of an analyte, as specified by an expert committee which
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must be detected and identified by the candidate method with a specified probability of
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identification (POI).
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Exclusivity
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Study involving pure non-target strains and species, which are potentially cross-reactive,
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that shall not be detected or identified by the test method.
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Inclusivity
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Study involving pure target strains or species that shall be detected and identified by the
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alternative method.
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Maximum Time-To-Assay Result
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Maximum time to complete an analysis starting from the test portion preparation to assay
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result.
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Probability of Identification (POI)
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The proportion of positive analytical outcomes for an identification method for a given
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matrix at a given analyte level or concentration.
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Venezuelan Equine Encephalitis(VEE) Virus
(VEEV)
VEEV encompasses several viruses all
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of which are within the Alphavirus genus of the
Togaviridae
family. For the purpose of this
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SMPR VEEV includes the human pathogenic virus variants VEEV-1AB, VEEV-1C, VEEV-1D, and
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VEE-1D.
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5.
System suitability tests and/or analytical quality control:
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The controls listed in Table I shall be made available in assays as appropriate. Manufacturer
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must provide written justification if controls are not available with the assay.
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