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DRAFT AOAC SMPR 2015.XXX; Version 7; June 16, 2015.
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Method Name:
Determination of Catechins, Methyl Xanthines, Theaflavins, and
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Theanines in Tea Dietary Ingredients And Supplements
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Purpose:
AOAC SMPR’s describe the minimum recommended performance characteristics to be
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used during the evaluation of a method. The evaluation may be an on-site verification, a single-
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laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by
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AOAC Stakeholder Panels composed of representatives from the industry, regulatory
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organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC
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SMPRs are used by AOAC Expert Review Panels in their evaluation of validation study data for
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method being considered for
Performance Tested Methods
or AOAC
Official Methods of
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Analysis
, and can be used as acceptance criteria for verification at user laboratories.
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Approved by:
Stakeholder Panel on Dietary Supplements (SPDS)
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Intended Use
: Quality control, routine testing and dispute resolution.
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1.
Applicability
:
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Quantitative determination of catechins, methyl xanthenes, theaflavins and theanine in tea
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dietary ingredients and supplements.
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2.
Analytical Technique
:
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Any analytical technique(s) that measures the analytes of interest and meets the following
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method performance requirements is/are acceptable. It is acceptable to have a different
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analytical method for each class of analytes.
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3.
Definitions
:
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Catechins
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Catechin, epicatechin, epigallocatechin, catechin gallate, epicatechin gallate,
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epigallocatechin gallate, and gallocatechin. Refer to table 3 for IUAC nomenclature and CAS
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registry numbers. See figure 1 for chemical structures.
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Dietary Ingredients
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A vitamin; a mineral; an herb or other botanical; an amino acid; a dietary substance for use
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by man to supplement the diet by increasing total dietary intake; or a concentrate,
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metabolite, constituent, extract, or combination of any of the above dietary ingredients
. 238
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Dietary supplements
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A product intended for ingestion that contains a "dietary ingredient" intended to add
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further nutritional value to (supplement) the diet. Dietary supplements may be found in
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many forms such as tablets, capsules, softgels, gelcaps, liquids, or powders.
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Refer t
o Appendix F :Guidelines for Standard Method Performance Requirements
in the 19
th
Edition of the AOAC INTERNATIONAL
Official Methods of Analysis (2012).
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United States
Federal Food Drug and Cosmetic Act
§201(ff) [U.S.C. 321 (ff)]