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Abstract

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Keywords

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Description of Folin Ciocalteau Reagent

1

Chemistry controlling the reaction with Folin-Ciocalteau reagent

1

Summary of Manual Dual Reagent Procedure [2]

1

Summary of Single reagent Procedure [4, 5]

2

Specific antioxidant classes and compounds detected by the F-C reagent

2

Procedure for the dual and single reagent assay methods for comparison

2

Dual reagent assay

2

Standard Preparation

2

Standard Catechin Sample Preparation and Analysis

2

Standard Curve Determination.

2

Folin assay of polyphenolic compounds (see Table 1)

2

Single reagent assay

4

Standard Catechin Sample Preparation and Analysis.

4

Standard Curve Determination

5

Folin assay of polyphenolic compounds

5

Free Polyphenolic concentration of herbs and spices by single and dual reagent (Table 2)

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Comparison of the two methods for pure polyphenols and samples

6

Other Dual Reagent Applications

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Microplate reader for polyphenols [21]

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Instrument

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Reagent Setup

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Equipment Setup

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Procedure

8

Solid Phase Extraction Method to remove interferences [22]

7

Sample Preparation.

7

Material and Solvent Standards.

7

Preparation of Raw Extracts

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Separation of Polyphenol and Other Water-Soluble Components by Solid-Phase Extraction.

7

Elimination of Vitamin C from WE.

7

Folin-Ciocalteu Assay.

7

Determination of Polyphenols and Vitamin C and Expression of the Results.

7

Acid hydrolysis of herbs, fruits and vegetables for total polyphenol analysis [5]

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Procedure

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Polyclar procedure to remove interferences and measure free and total polyphenols

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Polyclar Procedure

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To measure free phenols

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To measure total phenols

8

Miscellaneous Methods

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Flow Injection Analysis

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Development of a mixed polyphenol standard

9

Urinary analysis of polyphenols

9

Other References

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References

9