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Analyte Definition
•
Description of the analyte:
Determination of content of Vitamins B
1
, B
2
,B
3
,and B
6
.
Total vitamin content consists of all free forms of each
nutrient and all common phosphorylated forms.
•
Rationale:
–
Common fortified forms are Thiamine chloride,
Riboflavin, Nicotinamide, and Pyridoxine.
–
Other common inherent forms are Thiamine mono, di
and triphosphate, FMN, FAD, Nicotinic Acid, Pyridoxal,
Pyridoxal 5’ phosphate, Pyridoxamine, and
Pyridoxamine 5’ phosphate.
Specific Performance Claims B
1
Analytical range
30 – 2000 μg /100 g
Limit of Quantitation (LOQ) ≤ 30*
Recovery
90-110%
Repeatability (RSDr)
≤ 5%
Reproducibility (RSDR) ≤10%