SPIFAN Stakeholder Panel (March 15, 2017)

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March 14, 2017
Summary of Method Validation Results for
Whey Protein Analysis Using SDS-CGE (2)
Parameter Acceptance criteria (SMPR) Results
Specificity – Matrix
Interference
Electropherograms from injections of purified water and
processed formulation matrix without protein ingredients
will be evaluated for presence of peaks at the migration
times corresponding to analyte proteins related peaks
No interfering peaks were
observed for purified water and
processed formulation matrix
Quantitation Limit ч10 whey protein g/100 g protein 20%
Linearity The R2 must be •0.99. The residuals on the residual plot
should be randomly distributed around zero.
Linear of R2 0.993-0.999 for the
area ratio of whey protein to casein
Logarithm of R2 0.993-0.996 for
whey protein as % of total protein.
Range Range – 20% to 80% of whey
protein in total protein in infant
formulas in tested linear range
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March 14, 2017
• Ping Feng, Wyeth Nutrition, 30F, CITIC Square, 1168 Nan Jing Rd. (W), Shanghai 200041, China
Email: ; phone: 18521400044
• Christophe Fuerer, Nestec SA, Lausanne, Switzerland; Email:
• Adrienne McMahon, Wyeth Nutrition, Askeaton, Ireland; Email:
Thank you!
The raw data of the SLV and the MLT of 4 Chinese labs are available upon request.
The SLV is published on J. AOAC, the electronic version can be found with link:
The manuscript is being printed in the Mar/Apr 2017 Journal issue, 16-0344.R1 - Quantification of whey protein
content in infant formulas by SDS-CGE (Sodium Dodecyl Sulfate – Capillary Gel Electrophoresis): First Action
2016.15 Single Lab Validation.