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DRAFT AOAC SMPR 2016.XXX; Version 4; December 9, 2015.
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Standard Method Performance Requirements for Determination of Ethanol in Kombucha
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Intended Use
: Use by trained technicians in a laboratory for routine quality assurance testing.
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1.
Applicability
:
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Determination of low levels of ethanol as expressed as alcohol by volume (ABV) in
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kombucha.
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2.
Analytical Technique
:
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Any analytical technique that meets the following method performance requirements is
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acceptable.
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3.
Definitions
:
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Alcohol by volume (%ABV)
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A standard measure of how much alcohol
(ethanol)is contained in a given volume of an
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alcoholic beverage(expressed as
a volume percent).
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Ethanol
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The 2-carbon alcohol with
a molecular formulaof CH
3
CH
2
OH. CAS Registry Number: 64-17-5
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Kombucha
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Kombucha is a fermented,
effervescent
tea beverage made by adding a symbiotic culture of
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bacteria and yeast (SCOBY) to a solution of tea and sugar, and may include other
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ingredients.
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Limit of Quantitation (LOQ)
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The minimum concentration which quantitative results may be obtained with 95%
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confidence.
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Repeatability
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Variation arising when all efforts are made to keep conditions constant by using the same
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instrument and operator and repeating during a short time period. Expressed as the
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repeatability standard deviation (SD
r
); or % repeatability relative standard deviation
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(%RSD
r
).
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Reproducibility
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The standard deviation or relative standard deviation calculated from among-laboratory
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data. Expressed as the reproducibility standard deviation (SD
R
); or % reproducibility relative
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standard deviation (% RSD
R
).
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Recovery Factor
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The fraction or percentage of the analyte that is recovered when the test sample is analyzed
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using the entire method.
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