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Submission Date
2016-09-16 11:49:10
Name
Melanie Downs
Organization
University of Nebraska-Lincoln
Title of Method
Detection and Quantification of Selected Food Allergens using LC-MS/MS
AOAC Candidate Method
Number (e.g. ALN-01)
ALL-01
Applicable SMPR
2016.002
Summary:
The authors have submitted a method which they claim detects and quantifies egg,
milk, peanut, and hazelnut in various food matrices, in accordance with AOAC SMPR
2016.002. However, the authors have left out significant information about how the
method performance was evaluated. It is also difficult to tell from the presentation of
the method whether the authors have an understanding of the core concepts of food
allergen detection.
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
Yes.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
It is unclear throughout the method whether the terms and units used by the authors
are the same as those outlined in the SMPR. In particular, the definition of the food
allergens in the SMPR does not seem to be consistently applied and/or the terms are
not described at all in the method. For example, the SMPR gives a definition for whole
egg, but the only material described in the method for egg, is purified egg albumin
(ovalbumin), and when the term “egg” is used throughout the method, it is unclear
exactly what the authors are referring to.
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes.
AOAC SPSFAM ERP REVIEW: MAIN FORM