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Do you recommend this
method be adopted as a First
Action and published in the
Official Methods of Analysis
of AOAC INTERNATIONAL?
Please specify rationale.
The proteomic approach described here and proposed by the authors uses LC–MS/MS
to specifically detect allergens in one analysis. Proteins unique to eggs, milk, peanut,
and hazelnut have been extracted, subjected to trypsin digestion and analysis by liquid
chromatography/triple quadrupole mass spectrometry, in order to find highly conserved
peptides that can be used as markers to detect components in the food. The
multiplexed LC/MS analytical method of detection of these marker peptides in foods
was based on retention time of analytes, accurate mass and product ions from MS/MS.
LC with electrospray ionization and tandem MS operating in the multiple reaction
monitoring mode provide high sensitivity and selectivity for trace analysis.
This multiplexed approach provides the means to test food for hidden allergenic
compounds with accuracy and sensitivity to satisfy both inspection and labelling
purposes. In my opinion base on my knowledge, I believe that the proposed method
might be considered as comprehensive LC/MS method for detecting and quantifying
peptide markers specific and unique to those proteins.
In my opinion, the AOAC Candidate Method #ALL-02 entitled ‘Multiplexed LC-MS
Method for the Detection and Quantitation of Selected Food Allergens (milk, hazelnut,
peanut and whole egg)’ described by Jennifer Sealey Voyksner, Jerry Zweigenbaum
and Robert Voyksner can be adopted in its present form as a First Action and
recommended for publication in the Official Methods of Analysis of AOAC
INTERNATIONAL.