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Submission Date
2016-09-16 12:40:37
Name
Linda Monaci
Organization
CNR-ISPA
Title of Method
Multiplexed LC-MS method for the detection and quantitation of selected food allergens
AOAC Candidate Method
Number (e.g. ALN-01)
ALL-02
Applicable SMPR
Yes
Summary:
The method submitted is a LC-MS/MS method for the multiplex detection and
quantitation of milk, hazelnut, peanut and egg in different food matrices. The method
proposed is based on protein extraction and subsequent digestion with three
proteolytic enzymes (pepsin, trypsin, chymotrypsin); the final digest is partly purified
before HPLC separation and MS/MS detection. The method was run on three different
MS instrument: 2 triple quadrupole Mass Spectrometers in MRM mode and on a Q-
TOF MS (selecting the precursor ion)
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
Yes, the applicability of the method is in line with applicability of the SMPR.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
Yes
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes general precautions to be taken are specified.
AOAC SPSFAM ERP REVIEW: MAIN FORM