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Submission Date
2016-09-15 11:03:13
Name
Rachel Stryffeler
Organization
The Coca-Cola Company
Title of Method
Ethanol Analysis in Kombucha Drinks
AOAC Candidate Method
Number (e.g. ALN-01)
KOM-03
Applicable SMPR
Standard Method Performance Requirements (SMPRs®) for Determination of Ethanol
in Kombucha
Summary:
This method uses headspace analysis of ethanol by gas chromatography-mass
spectrometry (GC-MS) for the quantitation of ethanol in kombucha. Ethanol is purged
from the diluted kombucha tea and concentrated on the trap, followed by separation
and detection by GC-MS. This method is highly suitable for this application, however
the report lacks some method and validation details.
1. Does the applicability of
the method support the
applicability of the SMPR? If not,
please explain what is missing.
GC-MS is a suitable technique for the quantitation of ethanol in liquid samples and is
commonly used for the quantitation of ethanol and other volatiles in water, beverages
and other liquid matrices.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
In the method itself, there is mention of the “Method Detection Limit (MDL)”, yet no
reference to the Limit of Quantitation (LOQ) specified by the SMPR. The “Calculation”
section of the method mentions “Quantitation Limit”, but only the lowest point of the
calibration curve is listed, which is not the true LOQ.
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes.
AOAC SPSFAM ERP REVIEW: MAIN FORM
I. Summary of Method
II. Review of Method Only