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Performance Characteristics
Analytical Range: 0.2 to 4.2 mg/L in measured solutions but could not be
determined per AOAC. LOQ: 0.2 mg/L Accuracy / Recovery: Recovery was 97%.
Could not be ascertained in the absence of cis‐isomer standards. Precision: RDS was
0.23%. Reproducibility: RSD <0.92%
System Suitability
Conditions not provided to allow suitability to be replicated.
—All instrument configuration; instrument details, including serial numbers and
acquisition information; and column details, including identification, were properly
recorded during laboratory operations. Equipment calibration, including signal
description and baseline noise determination, was performed before tests were
executed.
Do you recommend this method be
adopted as a First Action and
published in the Official Methods
of Analysis of AOAC
INTERNATIONAL? Please specify
rationale.
This is the basis of a functional method. Several things could improve and expand
the method. It may be more applicable to measuring free lutein and zeaxanthin than
the esters. I would recommend that some things be adjusted before recommending
first action status. Sample prep to address additional products. Assessment of the
composite‐specific absorbance. Inclusion of standards and beta‐cryptoxanthin.
Recommendation for the Method