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Question
Response
General
AOAC Method Number:
CHON-001
Method Title:
Selected Adulterants in Dietary Ingredients and Dietary
Supplements Containing Chodroitin Sulfate
Method Author(s):
United States Pharmaopeia
Reviewer Name:
Jana Hildreth
Summary of Method
Cellulose acetate membranes e;ectophoresis
with Toluidine Blue staining based on the
characteristic migration pattern
of chondroitin sulfate on cellulose acetate membranes
when subject to a voltaic current, producing visible
very well defined bands after staining the developed
membranes with a cationic dye (Toluidine Blue)
.
Method Scope / Applicability:
Screening method for selected adulterants in dietary
ingredients claiming to contain chondroitin sulfate:
Sodium hexametaphosphate
Sodium alginate Propylene glycol alginate sulfate sodium
Over-sulfated polysaccharides
General Comments About the
Method:
This method has a proven track record of finding
adulterants in chondroitin sulfate raw material and has
been in pharmacopieas worldwide.
Method Clarity
This is an extremely straight forward method that does
not require much training.
Pros/Strengths
The cost of running this method is very cheap. It has
proven to find new adulterants that more sophisticated
methods missed. The cost of the equipment is under
$2000 and training is minimal.
Cons/Weaknesses
Currently only a couple of labs have the set up to run this
method in the US.
Supporting Data
General Comments
I feel this is a great screening tool for incoming chodroitin
CHON-001