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Assay  

Synonymous with test or test method, e.g. enzyme 

immunoassay, complement fixation test or polymerase chain 

reaction tests.  AOAC defines an assay as the total of all of the 

steps from sample collection to final result.

Analyte

Constituent that is of interest in an analytical procedure.

Matrix

Totality of components of a material system except the 

analyte. Ex.: soil, water, air, etc.

Qualitative method

Analytical method that results in a binary result: present or 

absent.  Frequently, the method has a design cut‐off point at 

which the method is designed to be positive at all 

concentrations about the cut‐off point.

Q i

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uant tat ve met o

Analytical method that determines the absolute or relative 

abundance (often expressed as a concentration) of one, 

several, or all particular substance(s) present in a sample. 

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