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Method Review Form
AOAC INTERNATIONAL Standards Development
Name of Reviewer:
Yon-Hong Wang
Title of Method:
Quantitative and Qualitative Analysis of Mitragynine in "Kratom" (Mitragyna Speciosa)
by GC-MS, LC-MS/MS and UPLC-PDA
AOAC Candidate Method Number:
KRA-01
Applicable SMPR :
AOAC SMPR 2018.008
I.
SUMMARY OF METHOD
GC/MS, UPLC-PDA and LC-MS/MS methods were developed for qualitative and quantitative analysis
of mitragynine in Mitragyna speciosa (Kratom) and realted products. UPLC-PDA and LC-MS/MS
methods were validated by characterizing a dry leaf Kratom product. The developed methods were
applied for the analysis of small packets of drinks, capsules, tea leaves, powdered leaves and spent
leaves from a manufacturing processing facility.
II.
REVIEW OF THE METHOD ONLY:
1.
Does the applicability of the method support the applicability of the SMPR? If no, please explain
what is missing.
Yes.
2.
Does the analytical technique(s) used in the method meet the SMPR? If no, please specify what
how it differs from what is stated in the SMPR.
Yes.