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This is a very good research paper that indicated an important improvement of the

original Folin-C method. The original Folin-C method is good at testing hydrophilic

antioxidant capacity of a sample. However the revised method presented in this paper, if

the data presented are reliable, can test both hydrophilic and lipophilic antioxidant

capacity of a sample.This is an important improvement in the method accuracy.

However a SLV is required to 1) validate the method; and 2) obtain additional data as

required in the SMPR to support the

method.su

V. RECOMMENDATION FOR THE METHOD:

1.

Do you recommend this method be adopted as a First Action and published in the

Official

Methods of Analysis of AOAC INTERNATIONAL?

If no, please specify rationale.

This method is not ready to be adopted as a First Action although it is likely to have a

better accuracy in estimation of the total antioxidant capacity of a sample. An SLV is

required to validate the method.